Future Prospects

Fluorescence complementation

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Conclusions

Fos Jun Complementation is indeed happening. However, the induction of bacteria does not last a long time, breaks at room temperature within a few hours. Also it is not quite sure whether the cells are touching while the complementation takes place or are simply close to each other.

Future Work

• Mutational nalysis must be performed to turn our plasmids into BioBricks and this includes mutations of Pst and other sites.
• Work must be done to further understand the interesting formations we saw.
• More inductions must be done to understand exactly the fluorescence pattern.
• More understanding regarding stability of the protein products will be investigated.

Possible Future Work in the field

Fos and Jun complementation and half YFP can have a variety of applications. One that we were thinking about was to fuse either Fos or Jun with an element of the flagella and that would stick to the membrane of a next cell, hence forming a chain. By fusing to different membrane proteins, eventually one could use bacteria as building blocks for entire strucures that work in synchrony. What still has to be worked out is their short life span.