Biobrick delivery

From 2006.igem.org

Revision as of 16:10, 20 April 2006

One of the resources that MIT provides teams competing in iGEM is a copy of parts that have been submitted to the Registry. These parts also include those that were submitted as a result of previous iGEM competitions (a big reason why we want you to keep on top of [http://partsregistry.org/Help:How_to_send_parts sending] us your parts as you create them!). For iGEM 2006, the Registry will send out parts in the form of dry DNA.

Sometime in the next several weeks you will receive a package in the mail containing a plate that looks like this:

[Insert pic of last year's dry DNA]

What do you do with this plate once you have received it?

• The DNA at the bottom of the wells needs to be resuspended. To do this you must:

1. Peel off the foil cover OR puncture a whole through the foil with a pipette tip
2. Add X ul of TE Buffer (Tris-HCl?)
3. Take 1ul DNA and transform into your favorite competent cells, plate out on a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.
4. Pick a single colony and inoculate some broth (with the correct antibiotic) and grow ~18 hours.
5. Use the resulting culture to miniprep AND make your own glycerol stock (for further instruction on making a glycerol see [http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria this page]).

It is your DNA "sandbox" to play with.