Brown:Equipment needed

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lists can be create by using the asterix symbol, or the pound symbol for numbered lists,  
lists can be create by using the asterix symbol, or the pound symbol for numbered lists,  
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      $ 400.00   Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>  custom primers   
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$ 400.00
+
      $ 800.00   Antibodies for identifying cell division proteins 4 x $200   
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+
      $ 250.00   disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)   
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+
      $ 600.00   Gel kit: Agarose gel aparatus, power source   
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Primers for PCR ($16 x 25bp) Invitrogen.com custom primers
+
          TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros   
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+
          Taq polymerase kit, DNTP - Free bases (actg ready mixed)   
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      $ 600.00   Restriction enzymes  $60 x 10   
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      $ 300.00   Thermocycler   
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+
      $ 500.00   misc. equipment and supplies   
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$ 800.00
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      $ 4,000.00   DNA synthesis costs $1.40 per base.   
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        Protein coding regions are about 700 to 1000 bases long This budget is for 3 - 4 new proteins.
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+
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Antibodies for identifying cell division proteins 4 x $200
+
-
 
+
-
    
+
-
+
-
 
+
-
$ 250.00
+
-
+
-
 
+
-
disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)
+
-
 
+
-
    
+
-
+
-
 
+
-
$ 600.00
+
-
+
-
 
+
-
Gel kit: Agarose gel aparatus, power source
+
-
 
+
-
    
+
-
+
-
 
+
-
+
-
+
-
 
+
-
TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros
+
-
 
+
-
    
+
-
+
-
 
+
-
+
-
+
-
 
+
-
Taq polymerase kit, DNTP - Free bases (actg ready mixed)
+
-
 
+
-
    
+
-
+
-
 
+
-
$ 600.00
+
-
+
-
 
+
-
Restriction enzymes  $60 x 10
+
-
 
+
-
    
+
-
+
-
 
+
-
$ 300.00
+
-
+
-
 
+
-
Thermocycler
+
-
 
+
-
    
+
-
+
-
 
+
-
$ 500.00
+
-
+
-
 
+
-
misc. equipment and supplies
+
-
 
+
-
    
+
-
+
-
 
+
-
$ 4,000.00
+
-
+
-
 
+
-
DNA synthesis costs $1.40 per base.
+
-
 
+
-
    
+
-
+
-
 
+
-
+
-
+
-
 
+
-
Protein coding regions are about 700 to 1000 bases long
+

Revision as of 14:49, 17 May 2006

Jason, I just threw this in here from the budget, please feel free to edit it out and add your equipment list, cheers, --Johncumbers 10:48, 17 May 2006 (EDT) john

lists can be create by using the asterix symbol, or the pound symbol for numbered lists, 

      $ 400.00   Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>   custom primers   
      $ 800.00   Antibodies for identifying cell division proteins 4 x $200   
      $ 250.00   disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)   
      $ 600.00   Gel kit: Agarose gel aparatus, power source   
         TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros   
         Taq polymerase kit, DNTP - Free bases (actg ready mixed)   
      $ 600.00   Restriction enzymes  $60 x 10   
      $ 300.00   Thermocycler   
      $ 500.00   misc. equipment and supplies   
      $ 4,000.00   DNA synthesis costs $1.40 per base.   
        Protein coding regions are about 700 to 1000 bases long  This budget is for 3 - 4 new proteins.
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