Brown:Equipment needed

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{| border=1 cellspacing=0 cellpadding=5
{| border=1 cellspacing=0 cellpadding=5
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| '''Equipment'''
| '''Equipment'''
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| | type/size
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| quantity
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| freezers/fridges
| freezers/fridges
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| -80°C
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| incubator
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| 1
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| hot bath
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| cold bath
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| autoclave
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| cell shaker/incubator
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| source of ddH2O
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| hot plates w/ stirring
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| stir bars (set)
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| PCR thermocycler
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| microcentrifuge (w/temp cntrl.)
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| centrifuge
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| Electrophoresis gel set
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| Gel Power source
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| UV Gel viewer
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| UV Gel camera
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| Pipettemen
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| Electronic pipettor
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| bunsen burners
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| sparkers
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| wire loop
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| metal loop
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| Glassware
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| Bottles
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| Beakers
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| Centrifuge tubes
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| Graduated cylinders
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| Electronic pipette tips (packages)
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| Gloves (boxes)
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| Plasticware
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| centrifuge tubes
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| Pipette tips
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| Bacterial cell culture plates
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| bacterial cell culture tubes
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| -20°C
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| tube racks/holders
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| 1
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|-
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| | 4°C| 1|-
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| incubator | | 1|-
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| hot bath| | 1|-
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| cold bath| | 1|-
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| autoclave| | 1|-
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| cell shaker/incubator| | 1|-
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| source of ddH2O| | 1|-
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| hot plates w/ stirring| | 2|-
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| stir bars (set)| | 1|-
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| PCR thermocycler| | 1|-
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| microcentrifuge (w/temp cntrl.)| | 1|-
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| centrifuge | 50mL and 15mL| 1|-
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| Electrophoresis gel set| | 4|-
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| Gel Power source| | 1|-
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| UV Gel viewer| | 1|-
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| UV Gel camera| | 1|-
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| Pipettemen| | |-
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| | 20μL| 4|-
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| | 200μL| 4|-
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| | 1000μL| 4|-
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| Electronic pipettor| can use 1-25mL pipettes| 1|-
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| bunsen burners| | 2|-
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| sparkers| | 2|-
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| wire loop| to pick a bac clone| 3|-
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| metal loop| to spread bacteria| 3|-
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| | | |-
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| Glassware| | |-
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| | type/size| quantity|-
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| Bottles| | |-
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| | 1L| 4|-
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| | 500mL| 8|-
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| | 100mL| 8|-
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| Beakers| | |-
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| | 1L| 2|-
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| | 250mL| 8|-
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| Centrifuge tubes| | |-
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| | 50mL| 100|-
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| | 15mL| 100|-
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| Graduated cylinders| | |-
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| | 500mL| 2|-
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| | 100mL| 2|-
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| Electronic pipette tips (packages)| | |-
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| | 1mL| 2|-
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| | 5mL| 2|-
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| | 10mL| 2|-
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| | 25mL| 2|-
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| Gloves (boxes)| | |-
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| | small| 1|-
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| | medium| 4|-
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| | large| 4|-
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| | | |-
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| Plasticware| | |-
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| | | |-
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| centrifuge tubes| | |-
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| | 1.5mL| 2000|-
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| | 200μL (PCR)| 1000|-
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| Pipette tips| | |-
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| | 20μL| a lot|-
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| | 200μL| a lot|-
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| | 1000μL| a lot|-
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| Bacterial cell culture plates| | 200|-
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| bacterial cell culture tubes| | |-
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| | (polypropylene)| 100|-  
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| tube racks/holders| | |-
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| | for 1.5mL tubes| 4|-
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| for 200μL tubes| 2|-
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|  
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| Reagents
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| 70% ethanol
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|  
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|}
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Revision as of 22:21, 17 May 2006

Equipment type/size quantity
freezers/fridges
-80°C 1
-20°C 1
4°C| 1|- | 1|- | 1|- | 1|- | 1|- | 1|- | 1|- | 2|- | 1|- | 1|- | 1|- 50mL and 15mL| 1|- | 4|- | 1|- | 1|- | 1|- | |- 20μL| 4|- 200μL| 4|- 1000μL| 4|- can use 1-25mL pipettes| 1|- | 2|- | 2|- to pick a bac clone| 3|- to spread bacteria| 3|- | |- | |- type/size| quantity|- | |- 1L| 4|- 500mL| 8|- 100mL| 8|- | |- 1L| 2|- 250mL| 8|- | |- 50mL| 100|- 15mL| 100|- | |- 500mL| 2|- 100mL| 2|- | |- 1mL| 2|- 5mL| 2|- 10mL| 2|- 25mL| 2|- | |- small| 1|- medium| 4|- large| 4|- | |- | |- | |- | |- 1.5mL| 2000|- 200μL (PCR)| 1000|- | |- 20μL| a lot|- 200μL| a lot|- 1000μL| a lot|- | 200|- | |- (polypropylene)| 100|- | |- for 1.5mL tubes| 4|- 2|- Reagents 70% ethanol 
      $ 400.00   Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>   custom primers   
      $ 800.00   Antibodies for identifying cell division proteins 4 x $200   
      $ 250.00   disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)   
      $ 600.00   Gel kit: Agarose gel aparatus, power source   
         TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros   
         Taq polymerase kit, DNTP - Free bases (actg ready mixed)   
      $ 600.00   Restriction enzymes  $60 x 10   
      $ 300.00   Thermocycler   
      $ 500.00   misc. equipment and supplies   
      $ 4,000.00   DNA synthesis costs $1.40 per base.   
        Protein coding regions are about 700 to 1000 bases long  This budget is for 3 - 4 new proteins.
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