Brown:Equipment needed

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| '''Equipment'''
| '''Equipment'''
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| type/size
| type/size
| quantity
| quantity
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| 2
| 2
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|-  
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| sparkers| | 2|-  
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| sparkers
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| wire loop| to pick a bac clone| 3|-  
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|  
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| metal loop| to spread bacteria| 3|-  
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| 2
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| | | |-  
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|-  
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| Glassware| | |-  
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| wire loop
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| | type/size| quantity|-  
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| to pick a bac clone
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| Bottles| | |-  
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| 3
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| | 1L| 4|-  
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|-  
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| | 500mL| 8|-  
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| metal loop
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| | 100mL| 8|-  
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| to spread bacteria
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| Beakers| | |-  
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| 3
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| | 1L| 2|-  
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|-  
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| | 250mL| 8|-  
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|  
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| Centrifuge tubes| | |-  
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|  
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| | 50mL| 100|-  
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|-  
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| | 15mL| 100|-  
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| '''Glassware'''
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| Graduated cylinders| | |-  
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|-  
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| | 500mL| 2|-  
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|  
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| | 100mL| 2|-  
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| type/size
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| Electronic pipette tips (packages)| | |-  
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| quantity
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| | 1mL| 2|-  
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| Status
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| | 5mL| 2|-  
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|-  
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| | 10mL| 2|-  
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| Bottles
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| | 25mL| 2|-  
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|  
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| Gloves (boxes)| | |-  
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|  
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| | small| 1|-  
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|-  
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| | medium| 4|-  
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|  
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| | large| 4|-  
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| 1L
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| | | |-  
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| 4
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| Plasticware| | |-  
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|-  
-
| | | |-  
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|  
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| 500mL
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| 8
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|-  
 +
|  
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| 100mL
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| 8
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|-  
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| Beakers
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|  
 +
|  
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|-  
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|  
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| 1L
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| 2
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|-  
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|  
 +
| 250mL
 +
| 8
 +
|-  
 +
| Centrifuge tubes
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|  
 +
|  
 +
|-  
 +
|  
 +
| 50mL
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| 100
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|-  
 +
|  
 +
| 15mL
 +
| 100
 +
|-  
 +
| Graduated cylinders
 +
|  
 +
|  
 +
|-  
 +
|  
 +
|  
 +
500mL
 +
| 2
 +
|-  
 +
|  
 +
| 100mL
 +
| 2
 +
|-  
 +
| Electronic pipette tips (packages)
 +
|  
 +
|  
 +
|-  
 +
|  
 +
| 1mL
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| 2
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|-  
 +
|  
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| 5mL
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| 2
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|-  
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|  
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| 10mL
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| 2
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|-  
 +
|  
 +
| 25mL
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| 2
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|-  
 +
| '''Gloves (boxes)'''
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|-
 +
|  
 +
| type/size
 +
| quantity
 +
| Status
 +
|-  
 +
|  
 +
| small
 +
| 1
 +
|-  
 +
|  
 +
| medium
 +
| 4
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|-  
 +
|  
 +
| large
 +
| 4
 +
|-  
 +
|  
 +
|  
 +
|  
 +
|-  
 +
| Plasticware
 +
|  
 +
|  
 +
|-  
 +
|  
 +
|  
 +
|  
 +
|-  
| centrifuge tubes| | |-  
| centrifuge tubes| | |-  
| | 1.5mL| 2000|-  
| | 1.5mL| 2000|-  

Revision as of 22:34, 17 May 2006

Equipment
type/size quantity Status
freezers/fridges
-80°C 1
-20°C 1
4°C 1
incubator 1
hot bath 1
cold bath 1
autoclave 1
cell shaker/incubator 1
source of ddH2O 1
hot plates w/ stirring 2
stir bars (set) 1
PCR thermocycler 1
microcentrifuge (w/temp cntrl.) 1
centrifuge 50mL and 15mL 1
Electrophoresis gel set 4
Gel Power source 1
UV Gel viewer 1
UV Gel camera 1
Pipettemen
20μL 4
200μL 4
1000μL 4
Electronic pipettor can use 1-25mL pipettes 1
bunsen burner 2
sparkers 2
wire loop to pick a bac clone 3
metal loop to spread bacteria 3
Glassware
type/size quantity Status
Bottles
1L 4
500mL 8
100mL 8
Beakers
1L 2
250mL 8
Centrifuge tubes
50mL 100
15mL 100
Graduated cylinders

500mL

2
100mL 2
Electronic pipette tips (packages)
1mL 2
5mL 2
10mL 2
25mL 2
Gloves (boxes)
type/size quantity Status
small 1
medium 4
large 4
Plasticware
| |- 1.5mL| 2000|- 200μL (PCR)| 1000|- | |- 20μL| a lot|- 200μL| a lot|- 1000μL| a lot|- | 200|- | |- (polypropylene)| 100|- | |- for 1.5mL tubes| 4|- 2|- Reagents 70% ethanol 
      $ 400.00   Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>   custom primers   
      $ 800.00   Antibodies for identifying cell division proteins 4 x $200   
      $ 250.00   disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)   
      $ 600.00   Gel kit: Agarose gel aparatus, power source   
         TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros   
         Taq polymerase kit, DNTP - Free bases (actg ready mixed)   
      $ 600.00   Restriction enzymes  $60 x 10   
      $ 300.00   Thermocycler   
      $ 500.00   misc. equipment and supplies   
      $ 4,000.00   DNA synthesis costs $1.40 per base.   
        Protein coding regions are about 700 to 1000 bases long  This budget is for 3 - 4 new proteins.
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