Brown:Equipment needed

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| centrifuge tubes
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| 1.5mL
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| Pipette tips
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| | 1.5mL| 2000|-
 
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| | 200μL (PCR)| 1000|-
 
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| Pipette tips| | |-
 
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| | 20μL| a lot|-
 
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| Bacterial cell culture plates| | 200|-
 
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| bacterial cell culture tubes| | |-
 
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| | (polypropylene)| 100|-
 
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| tube racks/holders| | |-
 
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| | for 1.5mL tubes| 4|-
 
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| for 200μL tubes| 2|-
 
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| 200μL
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| 70% ethanol
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| 1000μL
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| Bacterial cell culture plates
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| 200
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| bacterial cell culture tubes
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| (polypropylene)
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| 100
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| tube racks/holders
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| for 1.5mL tubes
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| '''Reagents'''
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Revision as of 22:37, 17 May 2006

Equipment
type/size quantity Status
freezers/fridges
-80°C 1
-20°C 1
4°C 1
incubator 1
hot bath 1
cold bath 1
autoclave 1
cell shaker/incubator 1
source of ddH2O 1
hot plates w/ stirring 2
stir bars (set) 1
PCR thermocycler 1
microcentrifuge (w/temp cntrl.) 1
centrifuge 50mL and 15mL 1
Electrophoresis gel set 4
Gel Power source 1
UV Gel viewer 1
UV Gel camera 1
Pipettemen
20μL 4
200μL 4
1000μL 4
Electronic pipettor can use 1-25mL pipettes 1
bunsen burner 2
sparkers 2
wire loop to pick a bac clone 3
metal loop to spread bacteria 3
Glassware
type/size quantity Status
Bottles
1L 4
500mL 8
100mL 8
Beakers
1L 2
250mL 8
Centrifuge tubes
50mL 100
15mL 100
Graduated cylinders

500mL

2
100mL 2
Electronic pipette tips (packages)
1mL 2
5mL 2
10mL 2
25mL 2
Gloves (boxes)
type/size quantity Status
small 1
medium 4
large 4
Plasticware
type/size quantity Status
centrifuge tubes
1.5mL 2000
200μL (PCR) 1000
Pipette tips
20μL a lot
200μL a lot
1000μL a lot
Bacterial cell culture plates 200
bacterial cell culture tubes
(polypropylene) 100
tube racks/holders
for 1.5mL tubes 4
for 200μL tubes 2
Reagents
type/size quantity Status 
      $ 400.00   Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>   custom primers   
      $ 800.00   Antibodies for identifying cell division proteins 4 x $200   
      $ 250.00   disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)   
      $ 600.00   Gel kit: Agarose gel aparatus, power source   
         TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros   
         Taq polymerase kit, DNTP - Free bases (actg ready mixed)   
      $ 600.00   Restriction enzymes  $60 x 10   
      $ 300.00   Thermocycler   
      $ 500.00   misc. equipment and supplies   
      $ 4,000.00   DNA synthesis costs $1.40 per base.   
        Protein coding regions are about 700 to 1000 bases long  This budget is for 3 - 4 new proteins.
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