Brown:Equipment needed
From 2006.igem.org
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Revision as of 16:57, 19 May 2006
type/size | quantity | Have(x) | Location | Comments | |
Equipment | |||||
freezers/fridges | |||||
-80°C | 1 | ||||
-20°C | 1 | ||||
4°C | 1 | ||||
incubator (what kind?) | 1 | ||||
pH meter | 1 | ||||
hot bath | 1 | ||||
cold bath | 1 | ||||
autoclave | 1 | ||||
cell shaker inside 37 degree incubator | 1 | ||||
source of ddH2O | 1 | ||||
hot plates w/ stirring | 2 | ||||
stir bars (set) | 1 | ||||
PCR thermocycler | 1 | ||||
microcentrifuge (w/temp cntrl.) | temp. cntrl not essential | 1 | |||
centrifuge | 50mL and 15mL | 1 | |||
Electrophoresis gel set | 4 | ||||
Gel Power source | 1 | ||||
UV Gel viewer | 1 | ||||
UV Gel camera | 1 | ||||
Pipettemen | |||||
20μL | 4 | ||||
200μL | 4 | ||||
1000μL | 4 | ||||
Electronic pipettor | can use 1-25mL pipettes | 1 | |||
Manual pipettors / bulbs | 2 | ||||
bunsen burner | 2 | ||||
sparkers | 2 | ||||
wire loop | to pick a bac clone | 3 | |||
metal loop | to spread bacteria | 3 | |||
Glassware | |||||
Bottles | |||||
1L | 4 | ||||
500mL | 8 | ||||
100mL | 8 | ||||
Beakers | |||||
1L | 2 | ||||
250mL | 8 | ||||
Centrifuge tubes | |||||
50mL | 100 | ||||
15mL | 100 | ||||
Graduated cylinders | |||||
500mL | 2 | ||||
100mL | 2 | ||||
Electronic pipette tips (packages) | |||||
1mL | 2 | ||||
5mL | 2 | ||||
10mL | 2 | ||||
25mL | 2 | ||||
pasteur pipettes | 1 box | ||||
Gloves (boxes) | |||||
small | 1 | ||||
medium | 4 | ||||
large | 4 | ||||
Plasticware | |||||
eppendorf tubes | |||||
1.5mL | 2000 | ||||
200μL (thin wall for PCR) | 1000 | ||||
Pipette tips | |||||
20μL | a lot | ||||
200μL | a lot | ||||
1000μL | a lot | ||||
Bacterial cell culture plates | 200 | ||||
bacterial cell culture tubes | (polypropylene) | 100 | |||
tube racks/holders | |||||
for 1.5mL tubes | 4 | ||||
for 200μL tubes | 2 | ||||
Reagents | |||||
pH standard buffers for meter | 1 | ||||
antibiotics (amp, kan, etc) | various |