Brown:Summer workshops
From 2006.igem.org
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HOMEWORK!!! (this will make my workshop shorter) | HOMEWORK!!! (this will make my workshop shorter) | ||
| - | *1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid]. Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday). | + | *1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid]. Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th). |
*2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations. | *2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations. | ||
Revision as of 21:48, 19 May 2006
Monday June 5, 2006
Lab Safety
- clothing/protection
- common sense
- waste
- storage
Overview of research basics
- lab notebook
- resources
- why am I doing this experiment?
Making stock solutions
- pipetting
- weighing
- graduated cylinders
- volumetric flasks
- % solutions (vol/vol, g/vol)
- molar vs. normal solutions
- buffers
HOMEWORK!!! (this will make my workshop shorter)
- 1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid]. Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th).
- 2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations.
Led by: Ana (10am-12pm)
Lab safety training with engineering group from 1pm - 5pm
Please email Karen to join group. All students must have completed lab safety training to work in the lab.
Tuesday June 6, 2006
Restriction digest
Gel electrophoresis
Led by: Azeem and Jason
Wednesday June 7, 2006
Primer Design
PCR/Gel
Led by: Azeem and Jason
Thursday June 8, 2006
Bacterial cell culture
Bacterial transfection
Making plates
Led by: Jamie Gagnon
Friday June 9, 2006
Mini-prep
Led by: Azeem and Jason
