Answer T1
From 2006.igem.org
(Difference between revisions)
Revision as of 15:24, 30 May 2006
- For in vitro recombination, < 10 mM Mg++ and 30% glycerol traps reaction after DNA cleavage and 2 uM EtBr slows strand exchange.
- Doubtful that these conditions could be established in E coli.
