Answer T1

(Difference between revisions)

Revision as of 16:14, 30 May 2006

For in vitro recombination, < 10 mM Mg++ and 30% glycerol traps reaction after DNA cleavage and 2 uM EtBr slows strand exchange.
Unclear whether these conditions could be established in E coli.

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	# For in vitro recombination, < 10 mM Mg++ and 30% glycerol traps reaction after DNA cleavage and 2 uM EtBr slows strand exchange.		# For in vitro recombination, < 10 mM Mg++ and 30% glycerol traps reaction after DNA cleavage and 2 uM EtBr slows strand exchange.
-	# ~~Doubtful that~~ these conditions could be established in E coli.	+	# Unclear whether these conditions could be established in E coli.