Answer T1
From 2006.igem.org
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| - | + | For in vitro recombination, < 10 mM Mg++ and 30% glycerol traps reaction after DNA cleavage and 2 uM EtBr slows strand exchange. | |
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| + | Unclear whether these conditions could be established in E coli. | ||
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| + | In http://www.kip.uni-heidelberg.de/chromcon/publications/pdf-files/Rippe_NAR_97.pdf, superhelical density of a 1868 bp plasmid was -0.03, with 80% of plasmids having -6 supercoils. | ||
Revision as of 16:30, 30 May 2006
For in vitro recombination, < 10 mM Mg++ and 30% glycerol traps reaction after DNA cleavage and 2 uM EtBr slows strand exchange.
Unclear whether these conditions could be established in E coli.
In http://www.kip.uni-heidelberg.de/chromcon/publications/pdf-files/Rippe_NAR_97.pdf, superhelical density of a 1868 bp plasmid was -0.03, with 80% of plasmids having -6 supercoils.
