Brown:Summer workshops
From 2006.igem.org
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'''Thursday June 8, 2006''' | '''Thursday June 8, 2006''' | ||
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| + | Bacteria <Br> | ||
| + | A tentative list of topics:<Br> | ||
| + | 1. bacterial strains<Br> | ||
| + | 2. how to grow bacteria (solid/liquid culture) | ||
| + | <Br>3. how to avoid contamination | ||
| + | 4. how to grow plasmids within bacteria | ||
| + | 5. antibiotics resistance | ||
| + | 6. blue/white selection | ||
| + | 7. iGEM specific information | ||
Bacterial cell culture <Br> | Bacterial cell culture <Br> | ||
Bacterial transfection<Br> | Bacterial transfection<Br> | ||
Revision as of 19:21, 1 June 2006
Monday June 5, 2006
Lab Safety
- clothing/protection
- common sense
- waste
- storage
Overview of research basics
- lab notebook
- resources
- why am I doing this experiment?
Making stock solutions
- pipetting
- weighing
- graduated cylinders
- volumetric flasks
- % solutions (vol/vol, g/vol)
- molar vs. normal solutions
- buffers
HOMEWORK!!! (this will make my workshop shorter)
- 1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid]. Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th).
- 2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations.
Led by: Ana (10am-12pm)
Lab safety training with engineering group from 1pm - 5pm
Please email Karen to join group. All students must have completed lab safety training to work in the lab.
Tuesday June 6, 2006
Restriction digest
Gel electrophoresis
Led by: Azeem and Jason
Wednesday June 7, 2006
Primer Design
PCR/Gel
Led by: Azeem and Jason
Thursday June 8, 2006
Bacteria
A tentative list of topics:
1. bacterial strains
2. how to grow bacteria (solid/liquid culture)
3. how to avoid contamination
4. how to grow plasmids within bacteria
5. antibiotics resistance
6. blue/white selection
7. iGEM specific information
Bacterial cell culture
Bacterial transfection
Making plates
Led by: Jamie Gagnon
Friday June 9, 2006
Mini-prep
Led by: Azeem and Jason
