Brown:Summer workshops

From 2006.igem.org

Revision as of 19:22, 1 June 2006

Monday June 5, 2006

Lab Safety

• clothing/protection
• common sense
• waste
• storage

Overview of research basics

• lab notebook
• resources
• why am I doing this experiment?

Making stock solutions

• pipetting
• weighing
• graduated cylinders
• volumetric flasks
• % solutions (vol/vol, g/vol)
• molar vs. normal solutions
• buffers

HOMEWORK!!! (this will make my workshop shorter)

• 1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid]. Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th).
• 2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations.

Led by: Ana (10am-12pm)
Lab safety training with engineering group from 1pm - 5pm Please email Karen to join group. All students must have completed lab safety training to work in the lab.

Tuesday June 6, 2006

Restriction digest
Gel electrophoresis
Led by: Azeem and Jason

Wednesday June 7, 2006

Primer Design
PCR/Gel
Led by: Azeem and Jason

Thursday June 8, 2006

Bacteria
A tentative list of topics:
1. bacterial strains
2. how to grow bacteria (solid/liquid culture)
3. how to avoid contamination
4. how to grow plasmids within bacteria
5. antibiotics resistance
6. blue/white selection
7. iGEM specific information
Led by: Jamie Gagnon

Friday June 9, 2006

Mini-prep
Led by: Azeem and Jason