Davidson Parts
From 2006.igem.org
(Difference between revisions)
Revision as of 19:53, 6 June 2006
Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site.
Forward primer 5’ gcattctagaatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttacCagtgcaaattg 3’
Reverse primer 5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac 3’