Brown:TAE solution Protocols

From 2006.igem.org

Back

TAE SOLUTION PROTOCOL

I. 50X TAE STOCK SOLUTION

1. FOR EACH LITER OF SOLUTION: A.) 242 g. TRIS BASE B.) 57.1mL glacial acetic acid C.) 100mL 0.5M EDTA

2. Mix Tris with stir bar to dissolve in about 600mL of ddH20

3. Add EDTA and Acetic Acid

4. Bring final volume to 1L with ddH20

5. Store at room temp.

II. 1X TAE PROTOCOL

1. Add 10mL of 50X TAE to enough ddH2O to make 500mL solution