Pancake Parts

From 2006.igem.org

Hin gene: Part BBa_J31000

• Used Hin 100-1

Primers for Hin gene with LVA degredation tag

• Used HinLVA-2

Recombinational Enahancer: Part BBa_J3101

• used RE-11

Hix- Part BBa_J44000[http://partsregistry.org/Part:BBa_J44000]

• Used Hix C-2

Resistance Genes

• TetR Front - used TF10
• TetR Back- used TB5
• CmR Front - used CF4
• CmR Back - used CB8
• KanR Front - used KF7
• KanR Back - still trying to clone

Construction Intermediates

• Pbad-RBS - used culture 3
• Hix-TF-Hix-TT-RE - used culture 1
• Pbad-Hix-RBS - used culture 3
• TF-Hix-TT-RE - used culture 2

One Pancake Constructs