Construction Intermediates

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pBad Promoter- RBS BBa_J3102 [http://partsregistry.org/Part:BBa_J3102]

  • Used culture #3

pBad Promoter-Hix : BBa_S03529 [http://partsregistry.org/Part:BBa_S03529]

  • Used the Midi-prepped DNA that we recieved from the Mammoths

pLac Promoter-RBS : BBa_S03511 [http://partsregistry.org/Part:BBa_S03511]

  • Used culture #4

pBad Promotor-Hix-RBS (P-H-R) : BBa_S03530 [http://partsregistry.org/Part:BBa_S03530]

  • Used culture #3

Recombinational Enhancer- Double Forward terminator(RE-TT): BBa_S03512 [http://partsregistry.org/Part:BBa_S03512]

  • This part has a twin, S03513, that was made accidentally
  • Both parts were created on the registry by accident. It was never made in the laboratory.

Double Forward Terminator- Recombinational Enhancer (TT-RE) : BBa_S03516 [http://partsregistry.org/Part:BBa_S03516]

  • Used culture #1

HinLVA-Double Forward Terminator(HLVA-TT): BBa_S03514 [http://partsregistry.org/Part:BBa_S03514]

  • Used culture #1

Hin-Double Forward Terminator(H-TT): BBa_S03515 [http://partsregistry.org/Part:BBa_S03515]

  • Used culutre #3

Hix-Double Forward Terminator-RE(H-TT-RE) : BBa_S03522 [http://partsregistry.org/Part:BBa_S03522]

  • Used culture #1

TetR Forwards-HixC(TF-H): BBa_S03523 [http://partsregistry.org/Part:BBa_S03523]

  • Used culutre #2

TetR Forwards-Hix- Double Forward Terminator-RE(TF-H-TT-RE): BBa_S03524 [http://partsregistry.org/Part:BBa_S03524]

  • Used culture #2

Hix-TetR Forwards-Hix-Double Forward Terminator-RE(H-TF-H-TT-RE) : BBa_S03527 [http://partsregistry.org/Part:BBa_S03527]

  • Used culture # 2

TetR Backwards-Hix(TB-H) : BBa_S03525 [http://partsregistry.org/Part:BBa_S03525]

  • Used culture #4

TetR Backwards-Hix- Double Forward Terminator-RE(TB-H-TT-RE) : BBa_S03526 [http://partsregistry.org/Part:BBa_S03526]

  • Used culture # 3

Hix-TetR Backwards-Hix-Double Forward Terminator-RE(H-TB-H-TT-RE): BBa_S03528 [http://partsregistry.org/Part:BBa_S03528]

  • Used culture #3

TetR Backwards-RBS(backwards)(TB-RBSrev) : BBa_S03532 [http://partsregistry.org/Part:BBa_S03532]

  • Used culture #2

pBad-Hix C- TetR Backwards-RBS (backwards)(P-H-TB-RBSrev)  : BBa_S03533 [http://partsregistry.org/Part:BBa_S03533]

  • Used culture #1

TetR Backwards- RBS Backwards-HixC- Double Forwards Terminator-RE(TB-RBSrev-H-TT-RE) BBa_S03534 [http://partsregistry.org/Part:BBa_S03534]

  • We chose not to use this part for ligation.
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