Boston University 2006:making antibiotic stocks
From 2006.igem.org
Use the following protocol to produce 5 1mL aliquots of stock solution of your desired antibiotic. Use a 1:1000 dilution to reach a working concentration from the stock (i.e. take 5uL from the Amp stock and add to 5mL of LB broth to produce 100ug/mL Amp + LB broth).
Materials
- Powder of given antibiotic
- dH20 and/or 100% ethanol
- 5mL Falcon tube(s)
- 5 1.5mL microcentrifuge tubes (per 5mL falcon tube)
Method
| Antibiotic | Abrv. | Working | Stock | Notes | Color Code |
|---|---|---|---|---|---|
| Ampicillin | Amp | 100 ug/mL | 100 mg/mL | orange | |
| Chloramphenicol | Chm | 170 ug/mL | 170 mg/mL | in 100% ethanol | green |
| Kanamycin | Kan | 50 ug/mL | 50 mg/mL | red | |
| Tetracycline HCL | Tet | 50 ug/mL | 50 mg/mL | in 100% ethanol | yellow |
- a
- b
Notes
- Store all the aliquots at -20C.
- Tet is light-sensative, so be sure to prevent exposure (i.e. wrap it in tinfoil or keep it in a dark box)
