The 1st South American team synthetic machine

From 2006.igem.org

pSB1A3-PFe-mRFP : (PI + PII + BBa_J04450 )

SUMMARY

Escherichia coli strain ( DH5α ) was transformed with vector pSB1A3 containing promoters PI and PII from acidothiobacilus rus operon fused with device BBa_J04450 respectively , wich at 2545 bp (+) codes a monomeric red fluorescent protein mRFP. The pSB1A3-PFe- mRFP was standardized for its specific sensoribility response to iron ions, in order to detect iron contamination and corrosion. Iron (II) was used at different concentrations (0, 1, 50 and 100 ppm). The pSB1A3-Fe was grown under presence and absence of UV, oxygen, and different levels of pH. The biosensoribility was determined by the response of the pSB1A2-UV-Fe to the different concentrations of iron. This response was measured by presence/absence of fluorescence, meanwhile, foton-fenton interaction was measured by DNA concentration, bacterial growth and changes on growth medium (like pH, mV) . once performed, parameters were related to sensoribility of the 1st South American team synthetic machine.

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