Penn State University 2006

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The bacterial relay race takes advantage of an ability to control cellular motility using inducible promoters such as those involved in nutrient catabolism or quorum sensing. “Receiver” bacteria move in response to small-molecule signals either added to the system or originating from motile, “sender” strains. The most significant challenges relating to this project stem from difficulties of tightly controlling the target motility gene motB. Low levels of motB expression result in system failure (constitutive motility), and resolving this issue is essential to developing reliable modular systems that are the hallmark of synthetic biology.

>People

Undergraduate Team

  • [http://openwetware.org/wiki/IGEM:PennState/2006|Jon Badalamenti]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Lucien Weiss]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Chris Buckno]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Joel Q. Grim]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Ralph Sandoval]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Barbara Andersen]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Jen Dunning]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Natalie Shewski]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|John Bigus]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Aleksandr Lewicki]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Elizabeth Morin]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Yan Shipelskiy]

Affiliated Grad Students

  • [http://openwetware.org/wiki/IGEM:PennState/2006|Steve Walker]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Deepti Tanjore]

Faculty

  • [http://openwetware.org/wiki/IGEM:PennState/2006|Tom Richard]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Pat Cirino]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Will Hancock]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Darryl Farber]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Ming Tien]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Paul Weiss]

Graduated Team Members

  • [http://openwetware.org/wiki/IGEM:PennState/2006|Lane J. Weaver]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Tony Tascone]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Steve Neuman]

Photos

  • [http://openwetware.org/wiki/IGEM:PennState/2006|Lab Photos]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Team Member Photos]



>Project

Publications

  • [http://openwetware.org/wiki/IGEM:PennState/2006|Synthetic Sports: A Bacterial Relay Race]

Protocols

  • [http://openwetware.org/wiki/IGEM:PennState/2006|Cloning]
    • [http://openwetware.org/wiki/IGEM:PennState/2006|Preparing chemically competent cells]
    • [http://openwetware.org/wiki/IGEM:PennState/2006|Growing Parts]
    • [http://openwetware.org/wiki/IGEM:PennState/2006|Restriction]
    • [http://openwetware.org/wiki/IGEM:PennState/2006|Ligation]
    • [http://openwetware.org/wiki/IGEM:PennState/2006|Transformation]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Plate-reading]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Motility-assays]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Microchannel]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Strain Construction]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Inventory]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|DNA Gel]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Growth Media]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|LB Plates]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Eiken Agar Plates]

Progress

  • [http://openwetware.org/wiki/IGEM:PennState/2006|Submitted Registry Parts]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Strain-Construction Progress]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Microchannels results]
  • [http://openwetware.org/wiki/IGEM:PennState/2006|Motility results]

Background

Links

  • iGEM on OpenWetWare
  • [http://partsregistry.org Parts registry]
  • [http://2007.igem.org iGEM wiki]
  • [http://2006.igem.org/wiki/index.php/Penn_State_2005 Penn State iGEM 2005]
  • [http://2006.igem.org/wiki/index.php/Penn_State_University_2006 Penn State iGEM 2006]

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