User:Bhickey

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Brendan M. Hickey Brown University Class of 2008 Computational Biology

Contents

Week 1

Most of this week was spent getting familiar with protocol and stocking the lab.

Week 2

This week was spent training to be an RA for Summer@Brown

Week 3

I spent the majority of this week arranging for the ungoing survival of the magenetotactic bacteria. I prepared 2 liters of Wolfe's mineral solution - a witch's brew containing a dozen or so trace metals. This is enough solution to generate 400 L of grow media for the bacteria. The bacteria are currently growing in Professor Wessel's yeast incubator and should be ready for observation in the middle of next week.

On Friday, Peter and I transformed cell with all the parts needed for the "Sender Cell" and then some.

Week 4

Sunday night, we (again with Peter) scraped colonies from the plates prepared on Friday and prepared seven tubes for mini-prep. Monday morning, we mini-prep'ed six parts. The 7th wasn't needed and was instead observed under a microsocope. On Wednesday, I'll run gels on the mini-prep results to confirm that the plasmids are intact.

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