From 2006.igem.org

Contents 1 Module Description 2 Group Members 3 Group Meeting History 4 Possible Implementation 4.1 Two Regulators 4.1.1 "Basic" (Giorgia) 4.1.2 Antisense (Giorgia) 4.2 One Regulators 4.2.1 AraC (Giorgia) 4.2.2 "Lunatic Activator" (Christophe) 4.2.3 Zinc Finger YY1 (Hervé) 4.2.4 Secondary Metabolism (Christophe) 4.2.5 Fusion Protein (Hervé) 4.2.6 Bidirectional Promoter (Hervé)

Module Description

In a nutshell, this module has 2 system boundaries, both of which are characterized by PoPS. Its purpose is to take a single input PoPS and output 2 different PoPS. One of the outputs should be high and the other low when S is high and vice versa when S is low.

Group Members

• Christophe, Dominic (coordinator), Giorgia, Herve, Kristian, Zlatko

Group Meeting History

log 2005-08-17: Wednesday, ~13:00 @ polyterasse: Discussion of module, next steps, task distrib.

Possible Implementation

Two Regulators

In the strategies below, the input PoPS regulates two proteins, an activator and a repressor.

"Basic" (Giorgia)

Antisense (Giorgia)

One Regulators

In the implementations below, the input PoPs regulate the production of a sole protein that somehow acts as activator and repressor. With one regulator, degradation is perfectly uniform, which is good, but it might be harder to obtain a symmetrical activity, which is bad.

AraC (Giorgia)

"Lunatic Activator" (Christophe)

Zinc Finger YY1 (Hervé)

Secondary Metabolism (Christophe)

Fusion Protein (Hervé)

Bidirectional Promoter (Hervé)