Lab Work
From 2006.igem.org
7th July 2006
When bacteria with the lacZ gene inserted are present in a medium containing lactose, the pH does drop significantly.
6th July 2006
Unfortunately the colonies we plated on the 4th did not survive due to a problem with the competent cells we used, so today we repeated transforming and plating colonies containing the following parts:
| 9E | BBa_E0033 | LacZ alpha | Plate 2 | KanR |
| 1I | BBa_B0015 | Terminator | Plate 1 | AmpR |
| 7K | BBa_R0010 | IPTG responsive promoter | Plate 1 | AmpR |
| 3O | BBa_B0034 | RBS | Plate 1 | AmpR |
4th July 2006
We plated colonies containing plasmids with the following parts:
| 9E | BBa_E0033 | LacZ alpha | Plate 2 | KanR |
| 3P | BBa_0010 | Terminator | Plate 2 | AmpR |
| 7K | BBa_R0010 | IPTG responsive promoter | Plate 1 | AmpR |
[http://2006.igem.org/Standard_Protocols Standard Protocols]
[http://2006.igem.org/University_of_Edinburgh_2006 Main page]
