The What's and How's

From 2006.igem.org

• What is the list of DNA parts that we will need for the first stage, second stage, entire project?
  
• ERIN and SABRIYA Which DNA parts exist in the registry?Answer1More Parts
  
• ERIN and SABRIYAWhich DNA parts will have to be designed? Will they be synthesized or produced by PCR?
  

loxP sites Hin/Hix CRE gene Degradation Tags

• Which plasmids will be used from the registry?
  
• Where would biobricks be located?
  
• MALCOLM What is the protocol for assembly for the first stage (restriction digestions, ligations, transformations)?
  

1. http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents
2. http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos
3. http://www.bio.davidson.edu/courses/Molbio/Protocols/pcr.html Setting up PCR mixtures
4. [[http://www.bio.davidson.edu/courses/Molbio/Protocols/magnesium.html PCR and Mg²⁺ concentration]]
5. http://www.bio.davidson.edu/courses/Molbio/Protocols/pourgel.html Pouring an agarose gel
6. http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html Calculate MWs
7. http://www.bio.davidson.edu/courses/Molbio/Protocols/digestion.html Digest DNA with restriction enzymes
8. http://www.bio.davidson.edu/courses/Molbio/Protocols/clean_short.html Clean DNA (short protocol)
9. http://www.bio.davidson.edu/courses/Molbio/Protocols/gels2002/1kbladder.pdf 1kb MW markers
10. http://www.bio.davidson.edu/courses/Molbio/Protocols/SAP.html Shrimp Alkaline Phosphatase
11. http://www.bio.davidson.edu/courses/Molbio/Protocols/gelpure.html Gel Purification
12. http://www.bio.davidson.edu/courses/Molbio/Protocols/ligation.html Ligation Protocol
13. http://www.bio.davidson.edu/courses/Molbio/Protocols/Promegacompcells.pdf Transformation http://www.bio.davidson.edu/courses/Molbio/Protocols/transformation.html Short version
14. http://www.bio.davidson.edu/courses/Molbio/Protocols/miniprepPrmega.html Promega miniprep