Construction

From 2006.igem.org

October 18, 2006

Andy, Konstantin:

• Transformed and plated Q03400 (2005) (2006)
• Gel-extracted UT1 DEF with EcoRI/SpeI (2500-3000, 5000, 8000), 8000 band bright, digestion appeared incomplete
• Made A, K, AK plates
• Made o/n of Q04400 (2005) (2006) Q04510 (2005) (2006)
• Tested UT3 in DH5a with 0%-2% arabinose

To-Do List:

• Make o/n of Q03400 (2005) (2006)
• Miniprep Q04400 (2005) (2006) and Q04510 (2005) (2006)
• Replate UT1 with miniprep from a good conlony
• Miniprep Q04400 (2005) (2006) Q04510 (2005) (2006) and check to see if bands are correct. If so, digest with EcoRI/XbaI and gel extract
• Ligate with UT1

[http://2006.igem.org/University_of_Toronto_2006 Home]

October 14, 2006

Charles, Jovan:

• Mini-prepped UT2 2/4 and UT3 7
• Transform UT2 2/4 and UT3 7 into DH5a-z1 and DH5a cells
• Continue with the IPTG test for the rest of the colonies that we weren’t able to test yesterday

To-Do List:

• Measure absorbance spectrum of Arabinose
• Make o/n of working UT2/UT3 for repeat of IPTG test.
• Look into using tetR and tet pL instead of cI and Prm+

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