Phase 1: PCR Amplification of Genes

From 2006.igem.org

A. Isolating genes for the quorum sensing mechanism from genomic B. subtilis DNA

      1. ComX - pheromone, 168bp
           Primers:  Fwd = CGGAATTCGCTCTAGATGCAAGACCTAATTAACTACTTTTTAAATTATCC
                     Rev = GACTGCAGCTACTAGTTAATCACCCCATTGACGGGTTATTGG
           Notes:  
           Forward primer contains EcoRI and XbaI cut sites; Reverse primer contains SpeI and PstI cut sites
           Amplification of ComX alone was unsuccessful.  However, ComQ and ComX are adjacent on the genome so we subsequently amplified them together