McGill University 2006
From 2006.igem.org
Contents |
Name: Fousion
News
- 2006-04-14 Discussion page created
- 2006-03-16 First kick-off meeting and team selection
If there is a specific task that we need the bacteria to do (e.g. change colour on cue, aggregate...) list it here. We have a TON of composite bricks, and can plan custom ones. BUT I need to know what U want... I am going nuts looking at the list
Your Dyslexic Dinosaur
Organisation
People
Students
Supervisors
| Jay Nadeau |
Timeline
Tasks
Parts shopping list
Composite bricks - these are the best to use as less cloning is required.
All these bricks express YFP and downregulate it in response to a signal
YELLOW
ARABINOSE RESPONSE.
BBa_E0610: Arabinose turns on lac repressor which in turn shuts down Yellow fluorescent protein.
BBa_E0600: like BBa_E0610 but YFP is destabilized - slow degradation. Not listed as available.
BBa_E0611: Arabinose turns on destabilized TetR repressor which shuts down yellow fluorescent protein. Addition of Tetracyline blocks TetR repressor allowing yellow fluorescent protein to be turned back on.
BBa_E0601: Like BBa_E0611 but YFP is destabilized - slow degradation. Not listed as available
BBa_E0613: Arabinose turns on destabilized lambda repressor which shuts down yellow fluorescent protein.
BBa_E0602: Like BBa_E0613 but YFP is destabilized - slow degradation. Not listed as available
BBa_E0603: Like BBa_E0602 but baseline expression of YFP is higher. Not listed as available
BBa_E0604: Arabinose turns on destabilized 434 cl epressor which in turn shuts down destabilized (slow) Yellow fluorescent protein. Not listed as available
BBa_E0614: Like BBa_E0604 but YFP is not destabilized (hangs around forever). Not listed as available
BBa_E0605: Arabinose turns on destabilized p22 repressor which in turn shuts down destabilized (slow) Yellow fluorescent protein. Not listed as available
BBa_E0615: Like BBa_E0605, but YFP is not destabilized, hangs around forever.
Lac I REGULATED
BBa_I12026: Produces YFP (not destabilized). Controlled by Lac I
MULTIPLE REGULATORS
BBa_I12051: Lactose induces lambda repressor. Arabinose induces 434 repressor. Lambda repressor activates YFP, 434 represses YFP. YFP is destabilized (AAV, fast).
BBa_I12052: Like BBa_I12051 but promoter for YFP has double binding sites for better control.
CYAN
TET response
BBa_I0401: Expresses CFP and lux from the Tet-OFF promoter. Neither is destabilized. Not listed as available
BBa_I0402: Like BBa_I0401 but lux is destabilized (LVA, slow)
BBa_I0404: Produces rhlR and CFP, downregulated by Tet. rhlR binds with N-butyryl-HSL, regulates transcription. rhlR but not CFP is destabilized.
BBa_I0405: same as BBa_I0404, but produces rhIl which produces N-butyryl-HSL.
BBa_I0406: produces CFP and lasR, which accepts chemical signal AI-1. Downregulated by Tet. LasR but not CFP are destabilized.
BBa_I0407. Like BBa_I0406 but produces lasI (destabilized LVA slow) which generates the chemical signal Al-1.
BBa_I0408: produces CFP and CinR, repressed by Tetracycline. Cin R is LVA (slow) destabilized. Cin R binds O3-C14:1-HSL and activates the Cin promoter.
BBa_I0409: Like BBa_I0408 but produces cinL (destabilized LVA slow) which generates the chemical signal O3-C14:1-HSL.
ARABINOSE response:
BBa_I0403 Expresses CFP and aiiA from an arabinose promoter. aiiA degrades N-acyl homoserine lactones (quorum sensing autoinducers). aiiA but not CFP is destabilized (LVA, slow).
SIGNALING
BBa_I0460 aiiA cassette. Produces enzyme which degrades N-acyl homoserine lactones (quorum sensing autoinducers. Destabilized with LVA (slow) B.Y.O.P (Bring your own promoter).
BBa_I10100: Produces adhesin, makes bacteria stick to each other. B.Y.O.P (Bring your own promoter)
BBa_I12002: Lambda promoter driving expression of 434 repressor. Not destabilized.
BBa_I12011: Lambda promoter driving expression of p22 repressor. LVA (slow) destabilized.
=Introduction= Our brainstorming meeting on 3-16 gave us several preliminary ideas which we will explore via modeling and research
