From 2006.igem.org
| Equipment
| type/size
| quantity
|
| freezers/fridges
|
|
|
|
| -80°C
| 1
|
|
| -20°C
| 1
|
|
| 4°C
| 1
|
| incubator
|
| 1
|
| hot bath
|
| 1
|
| cold bath
|
| 1
|
| autoclave
|
| 1
|
| | 1|-
| | 1|-
| | 2|-
| | 1|-
| | 1|-
| | 1|-
| 50mL and 15mL| 1|-
| | 4|-
| | 1|-
| | 1|-
| | 1|-
| | |-
| 20μL| 4|-
| 200μL| 4|-
| 1000μL| 4|-
| can use 1-25mL pipettes| 1|-
| | 2|-
| | 2|-
| to pick a bac clone| 3|-
| to spread bacteria| 3|-
| | |-
| | |-
| type/size| quantity|-
| | |-
| 1L| 4|-
| 500mL| 8|-
| 100mL| 8|-
| | |-
| 1L| 2|-
| 250mL| 8|-
| | |-
| 50mL| 100|-
| 15mL| 100|-
| | |-
| 500mL| 2|-
| 100mL| 2|-
| | |-
| 1mL| 2|-
| 5mL| 2|-
| 10mL| 2|-
| 25mL| 2|-
| | |-
| small| 1|-
| medium| 4|-
| large| 4|-
| | |-
| | |-
| | |-
| | |-
| 1.5mL| 2000|-
| 200μL (PCR)| 1000|-
| | |-
| 20μL| a lot|-
| 200μL| a lot|-
| 1000μL| a lot|-
| | 200|-
| | |-
| (polypropylene)| 100|-
| | |-
| for 1.5mL tubes| 4|-
| 2|-
|
|
| Reagents
| 70% ethanol
|
|
$ 400.00 Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/> custom primers
$ 800.00 Antibodies for identifying cell division proteins 4 x $200
$ 250.00 disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)
$ 600.00 Gel kit: Agarose gel aparatus, power source
TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros
Taq polymerase kit, DNTP - Free bases (actg ready mixed)
$ 600.00 Restriction enzymes $60 x 10
$ 300.00 Thermocycler
$ 500.00 misc. equipment and supplies
$ 4,000.00 DNA synthesis costs $1.40 per base.
Protein coding regions are about 700 to 1000 bases long This budget is for 3 - 4 new proteins.
