Brown:Equipment needed
From 2006.igem.org
| type/size | quantity | Status | ||||
| Equipment | freezers/fridges | |||||
| -80°C | 1 | |||||
| -20°C | 1 | |||||
| 4°C | 1 | |||||
| incubator | 1 | |||||
| hot bath | 1 | |||||
| cold bath | 1 | |||||
| autoclave | 1 | |||||
| cell shaker/incubator | 1 | |||||
| source of ddH2O | 1 | |||||
| hot plates w/ stirring | 2 | |||||
| stir bars (set) | 1 | |||||
| PCR thermocycler | 1 | |||||
| microcentrifuge (w/temp cntrl.) | 1 | |||||
| centrifuge | 50mL and 15mL | 1 | ||||
| Electrophoresis gel set | 4 | |||||
| Gel Power source | 1 | |||||
| UV Gel viewer | 1 | |||||
| UV Gel camera | 1 | |||||
| Pipettemen | ||||||
| 20μL | 4 | |||||
| 200μL | 4 | |||||
| 1000μL | 4 | |||||
| Electronic pipettor | can use 1-25mL pipettes | 1 | ||||
| bunsen burner | 2 | |||||
| sparkers | 2 | |||||
| wire loop | to pick a bac clone | 3 | ||||
| metal loop | to spread bacteria | 3 | ||||
| Glassware | ||||||
| type/size | quantity | Status | ||||
| Bottles | ||||||
| 1L | 4 | |||||
| 500mL | 8 | |||||
| 100mL | 8 | |||||
| Beakers | ||||||
| 1L | 2 | |||||
| 250mL | 8 | |||||
| Centrifuge tubes | ||||||
| 50mL | 100 | |||||
| 15mL | 100 | |||||
| Graduated cylinders | ||||||
|
500mL | 2 | |||||
| 100mL | 2 | |||||
| Electronic pipette tips (packages) | ||||||
| 1mL | 2 | |||||
| 5mL | 2 | |||||
| 10mL | 2 | |||||
| 25mL | 2 | |||||
| Gloves (boxes) | ||||||
| type/size | quantity | Status | ||||
| small | 1 | |||||
| medium | 4 | |||||
| large | 4 | |||||
| Plasticware | ||||||
| type/size | quantity | Status | ||||
| centrifuge tubes | ||||||
| 1.5mL | 2000 | |||||
| 200μL (PCR) | 1000 | |||||
| Pipette tips | ||||||
| 20μL | a lot | |||||
| 200μL | a lot | |||||
| 1000μL | a lot | |||||
| Bacterial cell culture plates | 200 | |||||
| bacterial cell culture tubes | ||||||
| (polypropylene) | 100 | |||||
| tube racks/holders | ||||||
| for 1.5mL tubes | 4 | |||||
| for 200μL tubes | 2 | |||||
| Reagents | ||||||
| type/size | quantity | Status |
$ 400.00 Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/> custom primers
$ 800.00 Antibodies for identifying cell division proteins 4 x $200
$ 250.00 disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)
$ 600.00 Gel kit: Agarose gel aparatus, power source
TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros
Taq polymerase kit, DNTP - Free bases (actg ready mixed)
$ 600.00 Restriction enzymes $60 x 10
$ 300.00 Thermocycler
$ 500.00 misc. equipment and supplies
$ 4,000.00 DNA synthesis costs $1.40 per base.
Protein coding regions are about 700 to 1000 bases long This budget is for 3 - 4 new proteins.
