Brown:Equipment needed
From 2006.igem.org
| type/size | quantity | Have(x) | Location | Comments | |
| Equipment | |||||
| freezers/fridges | |||||
| -80°C | 1 | ||||
| -20°C | 1 | ||||
| 4°C | 1 | ||||
| incubator (what kind?) | 1 | ||||
| pH meter | 1 | ||||
| hot bath | 1 | ||||
| cold bath | 1 | ||||
| autoclave | 1 | ||||
| cell shaker inside 37 degree incubator | 1 | ||||
| source of ddH2O | 1 | ||||
| hot plates w/ stirring | 2 | ||||
| stir bars (set) | 1 | ||||
| PCR thermocycler | 1 | ||||
| microcentrifuge (w/temp cntrl.) | temp. cntrl not essential | 1 | |||
| centrifuge | 50mL and 15mL | 1 | |||
| Electrophoresis gel set | 4 | ||||
| Gel Power source | 1 | ||||
| UV Gel viewer | 1 | ||||
| UV Gel camera | 1 | ||||
| Pipettemen | |||||
| 20μL | 4 | ||||
| 200μL | 4 | ||||
| 1000μL | 4 | ||||
| Electronic pipettor | can use 1-25mL pipettes | 1 | |||
| Manual pipettors / bulbs | 2 | ||||
| bunsen burner | 2 | ||||
| sparkers | 2 | ||||
| wire loop | to pick a bac clone | 3 | |||
| metal loop | to spread bacteria | 3 | |||
| Glassware | |||||
| Bottles | |||||
| 1L | 4 | ||||
| 500mL | 8 | ||||
| 100mL | 8 | ||||
| Beakers | |||||
| 1L | 2 | ||||
| 250mL | 8 | ||||
| Centrifuge tubes | |||||
| 50mL | 100 | ||||
| 15mL | 100 | ||||
| Graduated cylinders | |||||
|
500mL | 2 | ||||
| 100mL | 2 | ||||
| Electronic pipette tips (packages) | |||||
| 1mL | 2 | ||||
| 5mL | 2 | ||||
| 10mL | 2 | ||||
| 25mL | 2 | ||||
| pasteur pipettes | 1 box | ||||
| Gloves (boxes) | |||||
| small | 1 | ||||
| medium | 4 | ||||
| large | 4 | ||||
| Plasticware | |||||
| eppendorf tubes | |||||
| 1.5mL | 2000 | ||||
| 200μL (thin wall for PCR) | 1000 | ||||
| Pipette tips | |||||
| 20μL | a lot | ||||
| 200μL | a lot | ||||
| 1000μL | a lot | ||||
| Bacterial cell culture plates | 200 | ||||
| bacterial cell culture tubes | (polypropylene) | 100 | |||
| tube racks/holders | |||||
| for 1.5mL tubes | 4 | ||||
| for 200μL tubes | 2 | ||||
| Reagents | |||||
| pH standard buffers for meter | 1 | ||||
| 70% ethanol | |||||
| anitibiotics | |||||
| ampicillin | |||||
| kanamycin | |||||
| puromycin | |||||
| Agarose | |||||
| Ethidium Bromide | |||||
| tris[hydroxymethyl]aminomethane | |||||
| Acetate | |||||
| EDTA | |||||
| Gel Loading Dye | |||||
| DNA ladder | |||||
| Restriction enzymes and Buffers | |||||
| EcoRI | |||||
| SpeI | |||||
| XbaI | |||||
| PstI | |||||
| Taq Polymerase (Master Mix) | |||||
| 100% ethanol | |||||
| Socium Acetate | |||||
| NaCl | |||||
| T4 DNA ligase | |||||
| T4 DNA ligase Buffer | |||||
| liquid LB Broth | |||||
| LB with agar | |||||
| TSS Buffer | |||||
| Mini-Prep Kit | |||||
| Maxi-Prep Kit |
