Flip One Pancake

From 2006.igem.org

Flipping One Pancake

The plan is to construct a plasmid that contains the following:

1. Amp resistance gene as in pSB1A3
2. Hin recombinase gene driven by lacP (inducible in JM109 by IPTG or lactose), cloned from PCR product by Davidson team
3. RE (recombination enhancer), synthesized by Davidson team
4. One Pancake test construct:

araBAD promoter, inducible with arabinose
RBS
hixL recombination site, synthesized by Missouri Western team
CDS for mRFP in forward direction
hixR recombination site, synthesized by Missouri Western team
double forward terminator

To Do List

1. Plasmid prep