Davidson Parts

From 2006.igem.org

Hin gene: Part BBa_J31000

Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in bold.

Forward primer

5’ gcattctagaatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttacCagtgcaaattg 3’

Reverse primer

5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac 3’

Recombinational Enhancer: Part BBa_J3101

These are the oligos that we ordered to make the recombinational enhancer.

AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT

CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC

ATTTTACTAGTTGCGGCCGCCTGCA

GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA

AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG