Vector Digestions

From 2006.igem.org

Prepare linearized vector from pTet-GFP in the following two ways:

1. cut with XbaI + SpeI in order to receive PCR product of reversed pBAD
2. cut with EcoRI + PstI in order to receive annealed hix oligos

Each of the above should be done this way:

1. put together a restriction digestion using 400 ng vector and 40 ul volume
2. run on 1% agarose gel
3. purify the 2200 bp fragment (there will also be a smaller fragment from the pTet-GFP)