Cloning hix fragments

From 2006.igem.org

Set up four ligations as follows, with vector only and vector with each of the three annealed hix oligos from the right freezer:

4 ul of the purified EcorI/PstI digested vector from pTet-GFP 2 ul annealed hixL, hix R, hixC, or H2O 3 ul H2O 10 ul 2X Quick ligase buffer 1 ul Quick T4 ligase

room temp 5 min

Transform JM109 cells, along with the pAra-reverse ligations (take out one tube of 200 ul cells and divide it among the transformations, probably 200/6 = 33 ul each)