Vector Digestions

From 2006.igem.org

Prepare linearized vector from pTet-GFP in the following two ways:

1. cut with XbaI + SpeI in order to receive PCR product of reversed pBAD
2. cut with EcoRI + PstI in order to receive annealed hix oligos

Each of the above should be done this way:

1. put together a restriction digestion using 400 ng vector and 40 ul volume
2. run on 1% agarose gel
3. purify the 2200 bp fragment (there will also be a smaller fragment from the pTet-GFP)

(Todd -- I think the guys -- Brad, Eric, Kelly, Adam -- finished this this morning, but they did not document it. I was in class by the time they left and they are gone. My understanding is that they plan to have everyone here at noon tomorrow.)