University of Arizona 2006/Project Ideas

From 2006.igem.org

This page is part of the University_of_Arizona_2006 team site.

These ideas are under development

Contents 1 Jail Break/Treasure Hunt 1.1 Goals 1.2 Applications 1.3 Project Specifics 1.3.1 Switch States 1.3.2 Questions 1.4 Setup 1.5 Potential Parts and Devices 2 "Water Color" Painting 3 Cops and Robbers

Jail Break/Treasure Hunt

Goals

• Use a chemical attractant to move bacteria to a target
• Use a second attractant to move them away from the first target and to a second target
• Floresce when they reach each target

Applications

• Neat blinky bacteria
• Chemical finder
• Good use of a bistable switch
• more to come...

Project Specifics

Use a bistable switch to control the state of the cell.

Switch States

1. Attract to target 1
   
2. Attract to target 2

Flow: State 1 --> State 2 --> (halt)

Questions

• How do we start in state 1
• Are there more than one quorum sensing mechanisms (one for each state)

Setup

• Take a plate media and place two groups of stationary cells a and b.
• Each group of cells emit a different chemical attractant
• Take a third group of cells (the hunters) that use chemotaxis to move toward cell group or target a
• The hunters will group near the first target and activate a quorum mechanism

Quorum Mechanism:
* causes hunters to follow chemical attractant b
* causes first target to emit a florescent protien (or some visible beacon)

• The hunters now group at target b and activate a quorum mechanism

 TODO: Make a graphical representation of this process

Potential Parts and Devices

 TODO: Get list from Pat

"Water Color" Painting

 TODO: post description/setup of project

Cops and Robbers

This is has been put on the back burner