Davidson Assembly Plan

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Parts List

pLac promoter -- BBa_R0010 DNA-1 7K

pAra promoter -- BBa_I13453 DNA-2 13D

RBS -- BBa_B0030 DNA-1 3G

HixC – BBa_B?????

double forward terminator -- BBa_B0015 DNA-1 1I

double backward terminator – BBa_B0025 DNA-1 3E

KanR – BBa_B?????

Recombination enhancer -- BBa_J3101 (Davidson)

Hin -- BBa_J31000 (Davidson)

pAra promoter reverse – BBa_B????? (Western)

RBS reverse – BBa_B????? (Western)


Davidson will complete the TT-RE assembly below and send to Western.

TT-REJPEG.jpg

The Davidson cohort will also construct SuperParts necessary for the 1-pancake constructs in which the Kan resistance coding region is both forward and backward. Note: Davidson will need to send the forward KanR to Western for use in their assembly.

SuperPartCJPEG.jpg

SuperPartD1JPEG.jpg

SuperPartD2JPEG.jpg

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