Things That we Have Done

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Cloning

We have been able to successfully clone the following antibiotic pancakes

  • Hin
  • Hin with LVA degredation tag
  • Cmr- forward
  • Kan-forward
  • Tet-forward
  • Tet-backwards
  • Cmr-backwards
    • We are currently unsure if this is actually cloned due to the faint band of the insert. We are doing further studies of this to be sure that the insert is actually present. We have sent this to the Mammoths.

RE and Hix

We have been able to clone RE into cells. The RE that we are using has two point mutations but they should not compromise the activity of the Fis-binding sites please see the page for part Part BBa_J3101 [http://partsregistry.org/Part:BBa_J3101] for further details.

  • As per the [http://2006.igem.org/Davidson_Assembly_Plan| assembly plan] we will be attaching the RE downstream of the double terminator BBa_B0015 [http://partsregistry.org/Part:BBa_B0015]
  • We also have two cultures with the correct sequences for Hix C.
  • We have sent a "Master Plate" with cultures of all of the things that we have cloned to the Mammoths.
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