"E colock" Chiba 2006
From 2006.igem.org
Contents |
E colock "self-cycling-E. coli"
Member
Chiba 2006 http://chem.tf.chiba-u.jp/igem/ iGEM Chiba
『mu』team
Concept
By installing circadian rhythms of Cyanobacteria，E. coli starts oscillating.
How It Works
Installing Kai system
Kai system：the simplest and the most well known clock-system with a period of about 24h.
Kai system is driven by phosphorylation cascade composed of three proteins, such as KaiA,KaiB, and KaiC.
The oscillation of Kai system is converted to transcription cycle by Sas A.
This cycle is observable by produced fluorescent proteins.
Techinical Issues
1.Installing Kai Operon
The DNA is installable, whether the Kai system in cyanobacteria is function in E.coli or not?
2.Lack of Synchronization Mechanism
Cyanobacteria use light for “reseting” the cycle, thus its population keeps synchronizing.
However the genes for “reset bottom” has not been identified.
→→①Placing Lac represser under KaiA and KaiBC promoter,Kai oscillation is all stopped. The population is synchronized.
→→②Can we use AI??
Option
Control of cycle duration
There have been KaiA mutants with altered cycle periods. We want to do a similar to create “E clock” s with various cycle periods on demand.
Work Schedule and Progress
08.06 ①Seek for an appropriate promoter and fluoresceint protein for this project.←now
②Obtain KaiABC and SasA.
