Third Meeting, Wednesday, August 9, 2006
From 2006.igem.org
The Third Critical Design Review meeting took on 9th August, Wednesday at the SF Basement(SUDS).
Overview
4 basic endpoints: RFP, GFP, LacI and cI. Thus, the thermometer will be divided up into 4 basic modules.
Considerations
- Try to make parts that can be used to build other parts later (ie. Make a part and if test is successful, the part can then be used as is in the next ligation)
- Parallel Processing is KEY. Need to build as many different parts at the same time in order to use our time efficiently. We do not have time to wait and see if one part works before moving onto the next one. On that note, we will probably have to do at least two replicates per part (done by two different people) so that we have at least two cracks at each part
- Once a module is completed, there will first be a simple yes/no test to see if SOME output is produced. If yes, move onto building the second module. While the second module is being constructed, the first module will be more thoroughly tested for linear range, transcription rates, etc.
- All functionality of the parts is in context. One part may work fine in one context but not work in the next
Cell-See-Us Thermometer (2005 Team Design)
Top Level
Basically, LacI is produced, which dimerizes based on temperature: hot monomer, cold dimer. The dimer then inhibits the production of cI and RFP. cI is a transcription factor that when itself dimerizes, inhibits GFP. So, if cI is inhibited, GFP is produced. Therefore, the thermometer will glow green when cold, red and hot, and some fixed ratio of green:red in between.
Parts Level Expanded Form
Compressed Form
