Boston University 2006:making antibiotic stocks

From 2006.igem.org

Use the following protocol to produce 5 1mL aliquots of stock solution of your desired antibiotic. Use a 1:1000 dilution to reach a working concentration from the stock (i.e. take 5uL from the Amp stock and add to 5mL of LB broth to produce 100ug/mL Amp + LB broth).

Materials

• Powder of given antibiotic
• dH20 and/or 100% ethanol
• 5mL Falcon tube(s)
• 5 1.5mL microcentrifuge tubes (per 5mL falcon tube)

Method

Table of concentrations goes here

1. a
2. b

Notes

• Store all the aliquots at -20C.
• Tet is light-sensative, so be sure to prevent exposure (i.e. wrap it in tinfoil or keep it in a dark box)