Ljubljana, Slovenia 2006/Progress
From 2006.igem.org
We will report here about problems and successes in our lab work. Don't expect this to be really up to date, but we'll try to give some insight into how we proceed.
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Lab work started on June 7.
June - July: primer designs, vector minipreps, transformations, PCRs & control digestions.
Late July: each team member was assigned a list of constructs and deadlines for their completion.
August: first constructs were due August 15. They were finished towards the end of September.
September: several mutations detected in some of the constructs. Repeating joining parts into complex units. Focusing on crucial constructs with confirmed sequence. First transfection of human cell line on September 19.
