Toronto

From 2006.igem.org

Welcome to the Blue Water Wiki

Good news, there is an E coli strain availabe that contains Tn10 inserted into LacZ, meaning it is lacZ- and Tetracycline sensitive.

about the Tet operon and Tn10 http://www.biomedcentral.com/1471-2180/5/1 http://www.biomedcentral.com/1471-2199/3/13

-Hannah

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UPdate ~ 12 Oct 2005

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15:54, 3 Oct 2005 (EDT)15:54, 3 Oct 2005 (EDT)~~ Hi everyone, I made a biobrick version of my system, http://parts2.mit.edu/r/parts/partsdb/view.cgi?part_id=6153 It is part # BBa_J11030 I hope all the parts are available... Also very important NOTE that this system MUST MUST MUST be in LacZ- bugs! So lets try and get a hold of those if we want to make my system

Hannah

[http://pubs.acs.org/cgi-bin/archive.cgi/chreay/2000/100/i07/pdf/cr990115p.pdf Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes.]

Chem Rev. 2000 Jul 12;100(7):2705-38. Daunert S, Barrett G, Feliciano JS, Shetty RS, Shrestha S, Smith-Spencer W.

Discusses important biosensing systems, mainly in E. coli.

On another note, currently if anyone wants to post files (Powerpoint presentations, papers, etc.) the file must be available online somewhere and linked to from this Wiki. If you need a file to be posted online, feel free to email it to me at emanuelnazareth@hotmail.com and I'll get back to you with how to link to it.

~ Emanuel