ETH2006 xor
From 2006.igem.org
Contents |
XOR gate
The XOR gate's PoPs output activity should be correlated to the PoPs input activity as shown in the picture:
input A ^ | H D L | D D D | L D H +---------> input B output: High, Low, Dont care
implementation
current
Part to be used in the prototype system: [http://partsregistry.org/Part:BBa_J34200 BBa_J34200]
implementation alternatives
when we knew that we were going to implement an XOR, we came up with different artificial variants to implement them, without knowing whether such a system would exist in biology or not. while our biologists started searching the literature, we modelled the variants to see which we might want to favor.
here, we pick out 2 variants with correspoinding simulation results to give an idea of the process of development.
modeling
we are interested in the input/output behavior at steady state, that is when the system has reached equilibra and the concentrations of concerned species don't change anymore. since we have 2 inputs and 1 ouptut, simulation results will be illustrated as a surface plot, inputs on the x/y axes and output on the z axis.
ideal xor gate
the expected input/output behavior of an ideal xor gate looks like this (with less green being better):
note that the anterior low (blue) part has low inputs, the rear one has high inputs.
simulation results / sensitivity analysis
assembly procedure
test procedure
test results
parts
[http://partsregistry.org/Part:BBa_J34100 BBa_J34100]