The 1st South American team synthetic machine

From 2006.igem.org

The 1st South American team synthetic machine:

pSB1A3- P Fe- mRFP (PI + PII + BBa_J04450)

Vector: pSB1A2

SUMMARY

Escherichia coli strain ( DH5α ) was transformed with vector pSB1A3 containing promoters PI and PII from acidothiobacilus rus operon fused with device BBa_J04450, wich contains a monomeric red fluorescence protein (mRFP), respectively and to a fluorescent protein coding region of 2545 bp from 5´-3´. The pSB1A3- P Fe- mRFP was standardized for its specific sensoribility response to iron ions, in order to detect iron contamination and corrosion. Iron (II) was used at different concentrations (0, 1, 50 and 100 ppm). The pSB1A3-Fe was grown under presence and absence of UV, oxygen, and different levels of pH. The biosensoribility was determined by the response of the pSB1A2-UV-Fe to the different concentrations of iron. This response was measured by, presence of fluorescence, DNA concentration, bacterial growth and changes on growth medium . These parameters were related to sensoribility of the 1st South American team synthetic machine.