Aromatic Bacteria : Chiba 2006
From 2006.igem.org
Contents |
concept
Wow!! By lighting bacteria, a refreshing scent stars filling the room. In the dark, the light sensing E.coli will turn orenge. With the red light on, the color of E.coli fades out, and aroma is produced instead.
members
How it works
aroma
Firstly,we are going to make E.coli that smell good!
We are going to get gene that codes these products synthases.
[http://en.wikipedia.org/wiki/Carene Carene]
[http://en.wikipedia.org/wiki/Limonene Limonene]
[http://en.wikipedia.org/wiki/Pinene Pinene] [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=2411482 sequence]
[http://en.wikipedia.org/wiki/Linalool Linalool]
[http://en.wikipedia.org/wiki/Geraniol Geraniol]
[http://en.wikipedia.org/wiki/Farnesene Farnesene]
light receptor
Secondary,We are going to make a light receptor that react different wavelength.
PCB reacts redlight (that is about 660nm). If we change PCB to PΦB and PEB , the absorption wavelength shifts 15nm respectively.
These are a structure of chromophore.
carotenoid
We are going to use the EBIY. EBIY produces ricopen. That puts out orange color.
parts
- [http://partsregistry.org/Part:BBa_R0083 Part:BBa_R0083]
another works
We are also going to make these systems. File:Aroma pathway.jpg
papers
Aroma etc…
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15236249 Yasuo Yoshikuni(2004)-Mono and diterpene production in Escherichia coli.]
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15577217&dopt=Abstract Gisho Honda(2004)cDNA isolation and functional expression of myrcene synthase from Perilla frutescens.]
Light receptor etc…
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16458890&dopt=Abstract Takayuki Kohchi(2006)-Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli.]
[http://www.pnas.org/cgi/content/full/101/50/17334?ck=nck J.Clark Lagarias(2004)-Harnessing phytochrome's glowing potential]
carotenoid etc…
news
- 10/15--We succeeded a experiments of aroma! farnessene and pinene are a kind of sweet fragrance. Geraniol and pinene have a citrus flavor.
- 9/23--We succesed yesterday's experiment. The smell of geraniol that is produced E.coli was a kind of citrus.
- 9/22--We tested efficency of E.coli that makes geraniol.
- 9/21--We are trying to make E.coli that makes geraniol.
- 9/12--We got a gene that codes geraniol synthase.
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