ETH2006 and

From 2006.igem.org

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AND gate

The AND gate's PoPs output activity should be correlated to the PoPs input activity as shown in the picture:

 input A ^
         | L  D  H
         | D  D  D
         | L  D  L
         +--------->
            input B

 output: High, Low, Dont care

Implementation alternatives

Part to be used in the prototype system: [http://partsregistry.org/Part:BBa_J34100 BBa_J34100]

An AND gate produces an output only if both input signals are present. We want to implement such a “device” by using a suppressor tRNA. In our proposed model Signal 1 would lead to the transcription of the T7 RNA polymerase. However, an early stop codon was introduced into the coding sequence, leading to the expression of a truncated, non-functional protein. Signal 2, on the other hand, will lead to the expression of a suppressor tRNA. This tRNA recognizes the stop codon, preventing the end of translation and leading to incorporation of a glutamine into the nascent amino acid chain, so that translation can be completed. Consequently, a functional T7 RNA polymerase is present which will recognize its cognate promoter (T7 promoter) and therefore initiate expression of the downstream reporter gene (mCherry).

Modeling

AND-tRNA → simulation results / sensitivity analysis

Assembly procedure

Test procedure

Test results

Parts

[http://partsregistry.org/Part:BBa_J34100 BBa_J34100]

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