From 2006.igem.org

Meetings:
Friday, 5. August, 09:00 @ Christophe's bureau (CAB F61.2) Summary of the meeting

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Contents 1 Scope 2 People 3 Modules 3.1 Module Tree 3.2 cell division 3.3 oscillator 3.4 triggering by hand 3.5 interface 3.6 diviser / counter 3.6.1 4 genes counter 3.6.2 toggle switch 3.7 reporter 4 Discussions

Scope

This group is focussing on Ideas related to Counters and Oscillators.

People

People currently working on these Ideas:

• Urs A. Müller
• Jonas Nart
• Christophe Dessimoz

Please feel free to add your name.

Modules

We try to divide all the Ideas related to our scope in some 5 or 6 Modules. Our Goal on the one hand is to define the modules in a way that allows one to design, implement and test the individual modules independently. On the other hand, we also want to ensure that it is possible to connect them to build bigger components with more functionality.

Module Tree

----------------       ----------- 
|              |       |    |    |
|cell division |-----> |    |    |
|              |       |    |    |
----------------       |    |    | 
                       |----|    |     ---------------------
----------------       |    |    |     |                   |      ------------
|              |       |    |    |     |                   |      |          |
|  oscillator  |-----> |    |    |---->|  divisor/counter  |----->| reporter |
|              |       |    |    |     |                   |      |          |
----------------       |    |    |     |                   |      ------------
                       |----|    |     ---------------------
----------------       |    |    |
|  triggering  |       |    |    |
|   by hand    |-----> |    |    |
|              |       |    |    |
----------------       -----------
                        Interface

cell division

oscillator

triggering by hand

interface

diviser / counter

4 genes counter

All substances which are involved should be produced and decomposed quickly
S = concentration signal from the interface
R1 is produced by gene (1) and represses gene (0) and (3)
R2 is produced by gene (2) and represses gene (0) and (1)
R3 is produced by gene (3) and represses gene (1) and (2)
R0 is produced by gene (0) and represses gene (2) and (3)

                 
                     ---S---
     R1             /       \              R3
      \             |       |             /
       \/|____      |       |      ____|\/
    ___/  (1) |_____v__   __v_____| (3)  \___
       \  ____| = =           = = |____  /  
        \|      | |           | |      |/   
                | |           | |             
               R2 R3         R0 R1

               R3 R0         R1 R2
                | |           | |
        /|___   | |           | |  ____|\ 
    ___/  (2) |_=_=____   ____=_=_|(0=4) \___
       \  ____|     =       =     |____  /
       /\|          |       |          |/\
      /             |       |             \
     R2             \       /              R0
                     ---S---
  

toggle switch

reporter

This will probably be one or more colors to indicate the current state of the counter.

Discussions

We invite anyone to make comments and participate in discussions here