Brown:Equipment needed
From 2006.igem.org
type/size | quantity | Status | |
Equipment | |||
freezers/fridges | |||
-80°C | 1 | ||
-20°C | 1 | ||
4°C | 1 | ||
incubator | 1 | ||
hot bath | 1 | ||
cold bath | 1 | ||
autoclave | 1 | ||
cell shaker/incubator | 1 | ||
source of ddH2O | 1 | ||
hot plates w/ stirring | 2 | ||
stir bars (set) | 1 | ||
PCR thermocycler | 1 | ||
microcentrifuge (w/temp cntrl.) | 1 | ||
centrifuge | 50mL and 15mL | 1 | |
Electrophoresis gel set | 4 | ||
Gel Power source | 1 | ||
UV Gel viewer | 1 | ||
UV Gel camera | 1 | ||
Pipettemen | |||
20μL | 4 | ||
200μL | 4 | ||
1000μL | 4 | ||
Electronic pipettor | can use 1-25mL pipettes | 1 | |
bunsen burner | 2 | ||
sparkers | 2 | ||
wire loop | to pick a bac clone | 3 | |
metal loop | to spread bacteria | 3 | |
Glassware | |||
Bottles | |||
1L | 4 | ||
500mL | 8 | ||
100mL | 8 | ||
Beakers | |||
1L | 2 | ||
250mL | 8 | ||
Centrifuge tubes | |||
50mL | 100 | ||
15mL | 100 | ||
Graduated cylinders | |||
500mL | 2 | ||
100mL | 2 | ||
Electronic pipette tips (packages) | |||
1mL | 2 | ||
5mL | 2 | ||
10mL | 2 | ||
25mL | 2 | ||
Gloves (boxes) | |||
small | 1 | ||
medium | 4 | ||
large | 4 | ||
Plasticware | |||
centrifuge tubes | |||
1.5mL | 2000 | ||
200μL (PCR) | 1000 | ||
Pipette tips | |||
20μL | a lot | ||
200μL | a lot | ||
1000μL | a lot | ||
Bacterial cell culture plates | 200 | ||
bacterial cell culture tubes | (polypropylene) | 100 | |
tube racks/holders | |||
for 1.5mL tubes | 4 | ||
for 200μL tubes | 2 | ||
Reagents |