File registry

From 2006.igem.org

File Registry:Good Afternoon Everyone, I started this page to register our Plasmids Minipreps, Etc. to provide additional information other than just what's on the Sheet.

Complete Plasmids Box:

1A: This is a MIDIPREP of pGFP that was performed on Monday May 29 by Adam and Ashwin. This midiprep combined two seedings that were performed on the prior weekend. Both have been ran through the same midiprep column. The plasmid DNA here should be extremely concentrated as the maximm 50 ml of Culture was used. The total dissolving volume here is 200uL of dH2O. Unfortunately some product may hjave been lost during the last step of transferring the product from a big to a small centrifuge tube.

1B: NO LONGER GOOD. This is a MINIPREP product of MscL plasmid purified from a culture of Stable 3 bacteria by Jieun, Aaron and Brock on Tuesday May 30. The way this was done is a 5ml culture of Stable 3 was divided into 5 tubes and then pooled into 2 final product tubes, 1B and 1C. The DNA in this tube was initially dissolved in 50 uL of water. 15 uL of this DNA was removed for a restriction digest by XmnI on the same day, May 30 leaving 35um. Unfortunately the soluution is no longer good because 2um XmnI was accidentally added to this tube.

1C: Like 1B, this is the other half of the MINIPREP product of MscL plasmid purified from a culture of Stable 3 bacteria by Jieun, Aaron and Brock on Tuesday May 30. Please refer to 1B for the way this was done. It was dissolved in 50uL of dH2O and as of this date, nothing was removed from this solution. It is believed to be STILL GOOD.

1D